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The microbial communities of both kefirs vary significantly according to the types of substrates and origin of kefir grains giving both kefirs their distinctive sensory and functional properties

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Samples were incubated at 37 C for 10 min in the dark, then washed twice with RPMI 1640 medium, resuspended in fresh medium, and analyzed via flow cytometry using a BD FACSAria II cell sorter (BD Biosciences)

doi: 10.1038/sj.onc.1206197

doi: 10.3389/fimmu.2025.1713426 Received 26 September 2025 Revised 13 December 2025 Accepted 19 December 2025 Published 24 March 2026 Volume 16 - 2025 Edited by Daniel P